2.0 Methods
2.1 Search Strategy
Relevant articles were identified from two different databases (Web of
Science and ScienceDirect) using keywords: allergic inflammation AND
mast cell activation. All reported research studies that use in
vitro mast cells were included in this systematic review. Filter was
applied to include research articles in English that were published from
2018-2023. This study was conducted according to the
Preferred Reporting Items for
Systematic Reviews and Meta-analysis (PRISMA) 2020 guidelines. The last
search for relevant articles in all databases was performed on 15
December 2023.
2.2 Eligibility Criteria
For this systematic review, the inclusion criteria are (1) in
vitro mast cell studies, (2) secondary cell line culture studies, and
(3) allergy-related studies. Only published research articles from
2018-2023 were included. On the other hand, the exclusion criteria are
(1) animal work that do not involve any in vitro mast cell
studies, (2) only involve primary cell line culture studies, and (3)
full-text not accessible.
2.3 Study Appraisal and Selection
All the articles obtained from the databases using the specific keywords
were organized according to their titles, and duplicates were identified
by the same title, authors, and year of publication. The redundant
studies were removed, and the remaining articles were screened using the
pre-defined eligibility criteria. Firstly, the title and abstract of
each article were assessed by two reviewers independently. Those that
matched the eligibility criteria were then subjected to full-text
screening to further determine their relevance. Disagreements between
the two reviewers throughout the screening process were resolved by
consensus, and the reasons for excluding the articles were recorded.