7.0 Conclusion
Using secondary mast cell lines to conduct research in allergy has come a long way since the discovery of mast cells in 1863122. Today in modern research, the scientific community is able to utilize different mast cell lines to study the role of mast cell in an allergic response. This systematic review illustrated the popular secondary mast cell lines that are used to study the molecular and cellular mechanisms involved in functional studies in allergy diseases, and to determine potential protective compounds for allergic treatments (Figure 5). Therefore, some of these well-known cell lines such as RBL-2H3, HMC-1, and LAD2 cells have been proven to be an asset in unraveling the molecular complexity of allergy. However, every cell line will have its own drawbacks such as absence of high affinity IgE receptor and long doubling time. In addition, the differences in cell source and maintenance in culture may influence the epigenetic character of these cells which potentially lead to results variations and inconsistency across laboratories. Thus, researchers must take into consideration of these factors before selecting the appropriate mast cell line for their studies.
8.0 Availability of data and material
Data available on request from the authors.