7.0 Conclusion
Using secondary mast cell lines to conduct research in allergy has come
a long way since the discovery of mast cells in 1863122. Today in modern research, the scientific
community is able to utilize different mast cell lines to study the role
of mast cell in an allergic response. This systematic review illustrated
the popular secondary mast cell lines that are used to study the
molecular and cellular mechanisms involved in functional studies in
allergy diseases, and to determine potential protective compounds for
allergic treatments (Figure 5). Therefore, some of these well-known cell
lines such as RBL-2H3, HMC-1, and LAD2 cells have been proven to be an
asset in unraveling the molecular complexity of allergy. However, every
cell line will have its own drawbacks such as absence of high affinity
IgE receptor and long doubling time. In addition, the differences in
cell source and maintenance in culture may influence the epigenetic
character of these cells which potentially lead to results variations
and inconsistency across laboratories. Thus, researchers must take into
consideration of these factors before selecting the appropriate mast
cell line for their studies.
8.0
Availability of data and material
Data available on request from the authors.